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KMID : 0545120110210040387
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 4 p.387 ~ p.390
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Evaluation of the Potential Risk of Porcine Endogenous Retrovirus (PERV) Infection in Nude Mice
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Bae Eun-Hye
Jung Yong-Tae
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Abstract
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Nude mice (BALB/c) were grafted with human 293 cells and PERV (porcine endogenous retrovirus)-IRES-EGFP (a packageable retroviral vector plasmid containing an internal ribosome entry site-enhanced green fluorescent protein)-producing pig PK15 cells in order to determine whether the pig cells could transmit PERV-IRES-EGFP to mice and human 293 cells in vivo. None of the transplanted human 293 cell lines were infected by PERV, but PCR analysis identified PERV-B provirus integration into both the heart and salivary gland of the inoculated nude mice. Our data indicate that hearts and salivary glands can be used to identify PERV-B receptors.
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KEYWORD
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animal model, nude mice, porcine endogenous retroviruses, replication-defective retroviral vector, xenotransplantation
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